Antigen Measurement Using ELISA

The initial description of the enzyme-linked immunosorbent assay (ELISA) almost 30 yr ago (1) marked a technological advance that has had an immense impact in both clinical diagnostic and basic scientific applications. This assay represents a simple and sensitive technique for specific, quantitative...

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Published in: The Protein Protocols Handbook pp. 1827 - 1833
Main Author: Jordan, William
Format: Book Chapter
Language: English
Published: Totowa, NJ Humana Press
Series: Springer Protocols Handbooks
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Summary: The initial description of the enzyme-linked immunosorbent assay (ELISA) almost 30 yr ago (1) marked a technological advance that has had an immense impact in both clinical diagnostic and basic scientific applications. This assay represents a simple and sensitive technique for specific, quantitative detection of molecules to which an antibody is available. Although there are a huge number of variations based on the original ELISA principle, this chapter focuses on perhaps the two most useful and routinely performed: (1) the indirect sandwich ELISA—providing high sensitivity and specificity and (2) the basic direct ELISA—useful when only one antibody to the sample antigen is available.
ISBN: 160327474X
9781603274746
ISSN: 1949-2448
1949-2456
DOI: 10.1007/978-1-59745-198-7_194