Multi-modal nonlinear optical and thermal imaging platform for label-free characterization of biological tissue

The ability to characterize the combined structural, functional, and thermal properties of biophysically dynamic samples is needed to address critical questions related to tissue structure, physiological dynamics, and disease progression. Towards this, we have developed an imaging platform that enab...

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Published in: Scientific reports Vol. 11; no. 1; p. 8067
Main Authors: Adams, Wilson R, Mehl, Brian, Lieser, Eric, Wang, Manqing, Patton, Shane, Throckmorton, Graham A, Jenkins, J Logan, Ford, Jeremy B, Gautam, Rekha, Brooker, Jeff, Jansen, E Duco, Mahadevan-Jansen, Anita
Format: Journal Article
Language: English
Published: England Nature Publishing Group 04-13-2021
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Summary: The ability to characterize the combined structural, functional, and thermal properties of biophysically dynamic samples is needed to address critical questions related to tissue structure, physiological dynamics, and disease progression. Towards this, we have developed an imaging platform that enables multiple nonlinear imaging modalities to be combined with thermal imaging on a common sample. Here we demonstrate label-free multimodal imaging of live cells, excised tissues, and live rodent brain models. While potential applications of this technology are wide-ranging, we expect it to be especially useful in addressing biomedical research questions aimed at the biomolecular and biophysical properties of tissue and their physiology.
ISSN: 2045-2322
DOI: 10.1038/s41598-021-86774-2