Use of transforming growth factor‐β loaded onto β‐tricalcium phosphate scaffold in a bone regeneration rat calvaria model

Background Transforming growth factor‐β (TGF‐β1) enhances mesenchymal stem cell (MSC) differentiation into osteoblasts. Purpose The aim of the study was to assess whether TGF‐β1 loaded onto β‐tricalcium phosphate (β‐TCP) synthetic scaffold enhances bone regeneration in a rat calvaria model. The rele...

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Published in: Clinical implant dentistry and related research Vol. 21; no. 4; pp. 593 - 601
Main Authors: Elimelech, Rina, Khoury, Nizar, Tamari, Tal, Blumenfeld, Israel, Gutmacher, Zvi, Zigdon‐Giladi, Hadar
Format: Journal Article
Language: English
Published: Hoboken, USA John Wiley & Sons, Inc 08-01-2019
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Summary: Background Transforming growth factor‐β (TGF‐β1) enhances mesenchymal stem cell (MSC) differentiation into osteoblasts. Purpose The aim of the study was to assess whether TGF‐β1 loaded onto β‐tricalcium phosphate (β‐TCP) synthetic scaffold enhances bone regeneration in a rat calvaria model. The release kinetics of TGF‐β1 from β‐TCP scaffold was evaluated in vitro. Materials and Methods TGF‐β1 in various concentrations (1‐40 ng/mL) was loaded onto the β‐TCP scaffold, and release kinetics was monitored by ELISA. The effect of TGF‐β1 on the proliferation of MSCs was assessed using AlamarBlue, and MSC differentiation was evaluated by Alizarin Red quantification assay.Bone augmentation following transplantation of TGF‐β1 loaded onto β‐TCP in a rat calvaria model was evaluated in vivo. Results Greater TGF‐β1 release from the 40 ng/mL concentration was found. A suppressive effect of TGF‐β on the MSCs proliferation was observed with maximum inhibition obtained with 40 ng/mL compared to the control group (P = .028). A positive effect on MSCs osteogenic differentiation was found.Bone height and bone area fraction in vivo were similar with or without TGF‐β1; however, blood vessel density and degradation of the scaffold were significantly higher in the TGF‐β1 group. Conclusion TGF‐β1 adsorbed to β‐TCP stimulated angiogenesis and scaffold degradation that may enhance bone formation.
Bibliography: Funding information
Research Institute for Bone Repair, Rambam Health Care Campus, Haifa, Israel
ISSN: 1523-0899
1708-8208
DOI: 10.1111/cid.12775