Dynamics of Histone H3 Deposition In Vivo Reveal a Nucleosome Gap-Filling Mechanism for H3.3 to Maintain Chromatin Integrity

Establishment of a proper chromatin landscape is central to genome function. Here, we explain H3 variant distribution by specific targeting and dynamics of deposition involving the CAF-1 and HIRA histone chaperones. Impairing replicative H3.1 incorporation via CAF-1 enables an alternative H3.3 depos...

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Published in: Molecular cell Vol. 44; no. 6; pp. 928 - 941
Main Authors: Ray-Gallet, Dominique, Woolfe, Adam, Vassias, Isabelle, Pellentz, Céline, Lacoste, Nicolas, Puri, Aastha, Schultz, David C, Pchelintsev, Nikolay A, Adams, Peter D, Jansen, Lars E.T, Almouzni, Geneviève
Format: Journal Article
Language: English
Published: United States Elsevier Inc 2011
Elsevier B.V
Elsevier
Subjects:
DNA
RNA
Online Access: Get full text
Summary: Establishment of a proper chromatin landscape is central to genome function. Here, we explain H3 variant distribution by specific targeting and dynamics of deposition involving the CAF-1 and HIRA histone chaperones. Impairing replicative H3.1 incorporation via CAF-1 enables an alternative H3.3 deposition at replication sites via HIRA. Conversely, the H3.3 incorporation throughout the cell cycle via HIRA cannot be replaced by H3.1. ChIP-seq analyses reveal correlation between HIRA-dependent H3.3 accumulation and RNA pol II at transcription sites and specific regulatory elements, further supported by their biochemical association. The HIRA complex shows unique DNA binding properties, and depletion of HIRA increases DNA sensitivity to nucleases. We propose that protective nucleosome gap filling of naked DNA by HIRA leads to a broad distribution of H3.3, and HIRA association with Pol II ensures local H3.3 enrichment at specific sites. We discuss the importance of this H3.3 deposition as a salvage pathway to maintain chromatin integrity. [Display omitted] ► Failure in H3.1 deposition at replication sites permits replacement by H3.3 via HIRA ► RNA pol II presence correlates with H3.3 enrichment at genes and associates with HIRA ► DNA binding ability of HIRA reveals a gap-filling mechanism for H3.3 deposition ► HIRA-dependent H3.3 deposition as a salvage pathway to maintain chromatin integrity
ISSN: 1097-2765
1097-4164
DOI: 10.1016/j.molcel.2011.12.006